Invitrogen Cardiotrophin 1 (CTF1) Mouse ELISA Kit, 5 x 96 Tests

Invitrogen Cardiotrophin 1 (CTF1) Mouse ELISA Kit, 5 x 96 Tests

The Mouse Cardiotrophin 1 (CT-1) ELISA quantitates Ms CT-1 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms CT-1.

Principle of the method

The Mouse CT-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Homogenous (no wash): No
• Interassay CV: <12%
• Intraassay CV: <10%
• Shipping condition: Wet or Dry Ice
• Storage: 2-8°C

Contents

• Mouse CT-1 Antibody Coated wells, 96-well plate, 1 plate
• Mouse CT-1 Biotin Conjugate, 2 vials
• Mouse CT-1 Standard, recombinant mouse CT-1, 2 vials
• Wash Buffer Concentrate (20X), 25 mL
• Assay Diluent C, 30 mL
• Assay Diluent B (5X), 15 mL
• Streptavidin-HRP (200X), 0.2 mL
• TMB Substrate, 12 mL
• Stop Solution, 8 mL
• Adhesive Plate Covers, 2