Invitrogen CCL6 Mouse ELISA Kit, 96 Tests
On demandInvitrogen CCL6 Mouse ELISA Kit, 96 Tests
The Mouse CCL6 ELISA quantitates Ms CCL6 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms CCL6.
Principle of the method
The Mouse CCL6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: <12%
- Intraassay CV: <10%
- Shipping condition: Wet or Dry Ice
- Storage: 2-8°C
Contents
- Mouse CCL6 Antibody Coated wells, 96-well plate, 1 plate
- Mouse CCL6 Biotin Conjugate, 2 vials
- Mouse CCL6 Standard, recombinant mouse CCL6, 2 vials
- Wash Buffer Concentrate (20X), 25 mL
- Assay Diluent A (contains 0.09% sodium azide), 30 mL
- Assay Diluent B (5X), 15 mL
- Streptavidin-HRP (300X), 0.2 mL
- TMB Substrate, 12 mL
- Stop Solution, 8 mL
- Adhesive Plate Covers, 2
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