Invitrogen CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), PE-Cyanine 5, 100 Tests, eBioscience™
On demand
Order number :
15-1079-42
Invitrogen CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), PE-Cyanine 5, 100 Tests, eBioscience™
The eBioH4A3 monoclonal antibody reacts with human CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a highly glycosylated protein of approximately 110kDa. It is predominantly expressed intracellularly in the lysosomal/endosomal membrane in nearly all cells. CD107a is transiently expressed on the cell surface of degranulating cytolytic T cells, and is also upregulated on the surface of activated platelets and some cancer cells.
Features
- Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.
- Fixation: Samples can be stored in IC Fixation Buffer (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
- Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
- Filtration: 0.2 µm post-manufacturing filtered.
- Applications: Functional Assay (FN).
- Recommended Isotype Control: Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine5, eBioscience™.
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