Invitrogen CD11c Monoclonal Antibody (N418), APC-eFluor 780, 100 µg, eBioscience™

Invitrogen CD11c Monoclonal Antibody (N418), APC-eFluor 780, 100 µg, eBioscience™

The N418 monoclonal antibody reacts with mouse CD11c, the integrin alphaX. CD11c non-covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, a subset of Intestinal Intraepithelial Lymphocytes (IEL) and some activated T cells. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer.

• APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
• Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
• Fixation: Samples can be stored in IC Fixation Buffer (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
• Filtration: 0.2 µm post-manufacturing filtered.
• Recommended Isotype Control: Armenian Hamster IgG Isotype Control (eBio299Arm), APC-eFluor 780, eBioscience™.