Invitrogen CD127 Monoclonal Antibody (eBioRDR5), Super Bright 600, 100 Tests, eBioscience™
On demandInvitrogen CD127 Monoclonal Antibody (eBioRDR5), Super Bright 600, 100 Tests, eBioscience™
The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha). CD127 complexes with CD132, also known as the common gamma chain (gamma c), to form the multi-functional IL-7 receptor (IL-7R). CD127 is a type I glycoprotein with a molecular weight of 75-80 kDa and is expressed by immature B cells through the early pre-B stage, by thymocytes during several stages of their development, and on most mature T cells, with transient down-regulation upon activation. Binding of IL-7 results in signal transduction which occurs through several tyrosine kinase pathways including the Jak/STAT pathway. IL-7 is indispensible for the development of lymphocytes, and the control of homeostatic proliferation of T-cells in the periphery. In addition, IL-7R signaling is know to be involved in the regulation of T cell receptor (TCR) locus rearrangement in gamma delta T cells.
Features
- Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
- When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
- Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.
- Fixation: Samples can be stored in IC Fixation Buffer (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 2-8°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorochrome performance after fixation can be made, but clone specific performance should be determined empirically.
- Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser.
- Recommended Isotype Control: Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright 600, eBioscience™.
There are no specifications
There are no report
You May Also Like
Invitrogen CD80 (B7-1) Monoclonal Antibody (2D10.4), Functional Grade, 25 mg, eBioscience™
$ 10,212
On demand
Invitrogen CD80 (B7-1) Monoclonal Antibody (2D10.4), Super Bright™ 436, 100 Tests, eBioscience™
$ 398
On demand
Thermo Scientific™ LanthaScreen™ Tb-labeled Anti-mouse Ab, 1 mg
$ On demand
On demand
Invitrogen Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor 660, 25 µg, eBioscience™
$ On demand
On demand
Invitrogen Endomucin Monoclonal Antibody (eBioV.7C7 (V.7C7)), PE, 100 µg, eBioscience™
$ On demand
On demand
Invitrogen Mouse IgG2a kappa Isotype Control (eBM2a), APC-eFluor™ 780, 50 µg, eBioscience™
$ 202
On demand
Invitrogen 25 µg Rat IgG2a kappa Isotype Control (eBR2a), eFluor 660, eBioscience™
$ On demand
On demand
Invitrogen CD40 Monoclonal Antibody (1C10), Functional Grade, 50 µg, eBioscience™
$ On demand