Invitrogen CD14 Human ELISA Kit, 5 x 96 Tests
On demandInvitrogen CD14 Human ELISA Kit, 5 x 96 Tests
The Human CD14 ELISA quantitates Hu CD14 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CD14.
Principle of the method
The Human CD14 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: <12%
- Intraassay CV: <10%
- Storage: 2-8°C
Contents
- Human CD14 Antibody Coated wells, 96-well plate, 1 plate
- Human CD14 Biotin Conjugate, 2 vials
- Human CD14 Standard, recombinant human CD14, 2 vials
- Wash Buffer Concentrate (20X), 25 mL
- Assay Diluent (5X), 15 mL
- Streptavidin-HRP (400X), 0.2 mL
- TMB Substrate, 12 mL
- Stop Solution, 8 mL
- Adhesive Plate Covers, 2
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