Invitrogen CD16 Monoclonal Antibody (eBioCB16 (CB16)), Brilliant Ultra Violet™ 805, 100 Tests, eBioscience™
On demandInvitrogen CD16 Monoclonal Antibody (eBioCB16 (CB16)), Brilliant Ultra Violet™ 805, 100 Tests, eBioscience™
The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. CD16 is represented by two similar genes, CD16A (Fc gammaRIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fc gammaRIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.
Brilliant Ultra Violet™ 805 (BUV805) is a tandem dye that emits at 797
nm and is intended for use on cytometers equipped with an ultraviolet
(355 nm) laser. Please make sure that your instrument is capable of
detecting this fluorochrome.
When
using two or more Super Bright, Brilliant Violet™, Brilliant Ultra
Violet™, or other polymer dye-conjugated antibodies in a staining panel,
it is recommended to use Super Bright Complete Staining Buffer or Brilliant Stain Buffer to minimize
any non-specific polymer interactions. Please refer to the datasheet
for Super Bright Staining Buffer or Brilliant Stain Buffer for more
information.
Light
sensitivity: This tandem dye is sensitive to photo-induced oxidation.
Please protect this vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (100 µL
of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse
Solution for up to 3 days in the dark at 4°C with
minimal impact on brightness and FRET efficiency/compensation. Some
generalizations regarding fluorophore performance after fixation can be
made, but clone specific performance should be determined empirically.
Our
internal testing suggests that Brilliant Ultra Violet™ 805 (BUV805) is
compatible with short-term methanol-based fixation, but should not be
stored in buffers containing methanol for longer than one hour.
There are no specifications
There are no report
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