Invitrogen CD16 Monoclonal Antibody (eBioCB16 (CB16)), PerCP-eFluor™ 710, 100 Tests, eBioscience™
On demandInvitrogen CD16 Monoclonal Antibody (eBioCB16 (CB16)), PerCP-eFluor™ 710, 100 Tests, eBioscience™
The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. CD16 is represented by two similar genes, CD16A (Fc gammaRIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fc gammaRIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive photo-induced oxidation.
Please protect this vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (100 µL cell
sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4°C with minimal impact on
brightness and FRET efficiency/compensation. Some generalizations
regarding fluorophore performance after fixation can be made, but clone
specific performance should be determined empirically.
Filtration: 0.2 µm post-manufacturing filtered.
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