Invitrogen CD30L (TNFSF8) Mouse ELISA Kit, 5 x 96 Tests

Invitrogen CD30L (TNFSF8) Mouse ELISA Kit, 5 x 96 Tests

The Mouse CD30L (TNFSF8) ELISA quantitates Ms CD30L in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms CD30L.

Principle of the method

The Mouse CD30L solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Homogenous (no wash): No
• Interassay CV: <12%
• Intraassay CV: <10%
• Shipping condition: Wet or Dry Ice
• Storage: 2-8°C

Contents

• Mouse CD30L Antibody Coated wells, 96-well plate, 1 plate 
• Mouse CD30L Biotin Conjugate, 2 vials 
• Mouse CD30L Standard, recombinant mouse CD30L, 2 vials 
• Wash Buffer Concentrate (20X), 25 mL 
• Assay Diluent A (contains 0.09% sodium azide), 30 mL 
• Assay Diluent B (5X), 15 mL 
• Streptavidin-HRP (100X), 0.2 mL 
• TMB Substrate, 12 mL 
• Stop Solution, 8 mL 
• Adhesive Plate Covers, 2