Invitrogen CD31/PECAM (Soluble) Human ELISA Kit, 10 x 96 Tests

Invitrogen CD31/PECAM (Soluble) Human ELISA Kit, 10 x 96 Tests

The Human Soluble CD31/Platelet Endothelial Cell Adhesion Molecule(Hu sCD31/PECAM) ELISA quantitates Hu sCD31/PECAM in human serum, plasma, cell culture supernatants, urine, or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sCD31/PECAM.

Principle of the method

The Human sCD31/PECAM solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Homogenous (no wash): No
• Interassay CV: 7.4%
• Intraassay CV: 1.7%
• Storage: 2-8°C

Contents

• Pre-coated 96 well plate
• Standard
• Sample Diluent
• Assay Buffer concentrate
• HRP-conjugated Detection Antibody
• Wash Buffer
• Chromogen
• Stop Solution
• Controls
• Adhesive Plate Covers