Invitrogen CD3e Monoclonal Antibody (145-2C11), Functional Grade, 25 mg, eBioscience™

Invitrogen CD3e Monoclonal Antibody (145-2C11), Functional Grade, 25 mg, eBioscience™

The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.

Applications: ChIP assay (ChIP), Functional Assay (FN), Blocking Assay (BLOCK), T-Cell Activation (TCA) and In vitro Assay (IV).

Features

• Storage and handling: Use in a sterile environment.
• Filtration: 0.2 µm post-manufacturing filtered.
• Purity: Greater than 90%, as determined by SDS-PAGE.
• Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
• Aggregation: Less than 10%, as determined by HPLC.