Invitrogen CD4 Monoclonal Antibody (GK1.5), NovaFluor Blue 530, 25 µg, eBioscience™

Invitrogen CD4 Monoclonal Antibody (GK1.5), NovaFluor Blue 530, 25 µg, eBioscience™

The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.

NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.

Features  

• Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer. Use this buffer whenever staining with NovaFluor conjugates, including single-color compensation controls using cells. Use 5 µL of CellBlox Blocking Buffer per stained cell sample containing 10^3 to 10^8 cells.
• Excitation: 509 nm; Emission: 530 nm; Laser: 488 nm (Blue) Laser.