Invitrogen CD40 Mouse ELISA Kit, 10 x 96 Tests

Invitrogen CD40 Mouse ELISA Kit, 10 x 96 Tests

The Mouse CD40 ELISA quantitates Ms CD40 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms CD40.

Principle of the method

The Mouse CD40 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Homogenous (no wash): No
• Interassay CV: <12%
• Intraassay CV: <10%
• Shipping condition: Wet or Dry Ice
• Storage: 2-8°C

Contents

• Mouse CD40 Antibody Coated wells, 96-well plate, 10 plate 
• Mouse CD40 Biotin Conjugate, 20 vials 
• Mouse CD40 Standard, recombinant mouse CD40, 20 vials 
• Wash Buffer Concentrate (20X), 12 x 25 mL 
• Assay Diluent A (contains 0.09% sodium azide), 10 x 30 mL 
• Assay Diluent B (5X), 10 x 15 mL
• Streptavidin-HRP (200X), 10 x 0.2 mL 
• TMB Substrate, 10 x 12 mL 
• Stop Solution, 10 x 8 mL 
• Adhesive Plate Covers, 20