Invitrogen CD41a Monoclonal Antibody (eBioMWReg30 (MWReg30)), APC-eFluor 780, 100 µg, eBioscience™

Invitrogen CD41a Monoclonal Antibody (eBioMWReg30 (MWReg30)), APC-eFluor 780, 100 µg, eBioscience™

The eBioMWReg30 monoclonal antibody reacts with mouse CD41 (fibrinogen receptor, gpIIb, integrin alpha IIb). While initially thought to be expressed exclusively on the surface of platelets and megakaryocytes, it has been demonstrated that CD41 is also expressed on hematopoietic progenitors in the embryo, fetus and adult. CD41 associates with CD61 (gpIIIa, integrin beta III) to form a receptor which plays a major role in platelet function, including binding of several adhesion molecules such as fibrinogen, fibronectin and vitronectin.

Recently, the SLAM-family markers, CD48 and CD150 have been used to reliably identify hematopoietic stem cells (HSC). Specifically, it was found that CD150+CD48- bone marrow cells were highly efficient in their ability to confer long-term multi-lineage reconstitution in irradiated mice. Furthermore, the efficiency of reconstitution was enhanced when HSCs were further enriched through the exclusion of CD41+ cells. Thus, the use of CD150+CD48-CD41- as an expression profile efficiently identifies hematopoietic stem cells.

• APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
• Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
• Fixation: Samples can be stored in IC Fixation Buffer (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
• Filtration: 0.2 µm post-manufacturing filtered.
• Recommended Isotype Control: Rat IgG1 kappa Isotype Control (eBRG1), APC-eFluor 780, eBioscience™.