Invitrogen CD45 Monoclonal Antibody (30-F11), APC-eFluor 780, 100 µg, eBioscience™

Invitrogen CD45 Monoclonal Antibody (30-F11), APC-eFluor 780, 100 µg, eBioscience™

The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

• APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
• Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
• Fixation: Samples can be stored in IC Fixation Buffer (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
• Filtration: 0.2 µm post-manufacturing filtered.
• Recommended Isotype Control: Rat IgG2b kappa Isotype Control (eB149/10H5), APC-eFluor 780, eBioscience™.