Invitrogen CD62L (L-Selectin) Monoclonal Antibody (MEL-14), APC-eFluor 780, 25 µg, eBioscience™
On demand
Order number :
47-0621-80
Invitrogen CD62L (L-Selectin) Monoclonal Antibody (MEL-14), APC-eFluor 780, 25 µg, eBioscience™
The MEL-14 monoclonal antibody reacts with mouse CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCam-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.
Features
- APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
- Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
- Fixation: Samples can be stored in IC Fixation Buffer (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
- Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.
- Filtration: 0.2 µm post-manufacturing filtered.
- Recommended Isotype Control: Rat IgG2a kappa Isotype Control (eBR2a), APC-eFluor 780, eBioscience™.
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