Invitrogen CD71 (Transferrin Receptor) Monoclonal Antibody (OKT9 (OKT-9)), PerCP-eFluor 710, 25 Tests, eBioscience™

Invitrogen CD71 (Transferrin Receptor) Monoclonal Antibody (OKT9 (OKT-9)), PerCP-eFluor 710, 25 Tests, eBioscience™

The OKT9 monoclonal antibody reacts with human CD71, a 170-180 kDa type II transmembrane protein. CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71.

Features

• PerCP-eFluor 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
• Fixation: Samples can be stored in IC Fixation Buffer (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
• Recommended Isotype Control: Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor 710, eBioscience™.