Invitrogen CD80 (B7-1) Monoclonal Antibody (2D10.4), Brilliant Ultra Violet™ 737, 100 Tests, eBioscience™
On demandInvitrogen CD80 (B7-1) Monoclonal Antibody (2D10.4), Brilliant Ultra Violet™ 737, 100 Tests, eBioscience™
The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages and dendritic cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.
Brilliant Ultra Violet™ 737 (BUV737) is a tandem dye that emits at 732
nm and is intended for use on cytometers equipped with an ultraviolet
(355 nm) laser. Please make sure that your instrument is capable of
detecting this fluorochrome.
When using two or more Super Bright,
Brilliant Violet™, Brilliant Ultra Violet™, or other polymer
dye-conjugated antibodies in a staining panel, it is recommended to use
Super Bright Complete Staining Buffer or Brilliant
Stain Buffer to minimize any non-specific polymer
interactions. Please refer to the datasheet for Super Bright Staining
Buffer or Brilliant Stain Buffer for more information.
Light
sensitivity: This tandem dye is sensitive to photo-induced oxidation.
Please protect this vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (100 µL
of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse
Solution for up to 3 days in the dark at 4°C with
minimal impact on brightness and FRET efficiency/compensation. Some
generalizations regarding fluorophore performance after fixation can be
made, but clone specific performance should be determined empirically.
Our
internal testing suggests that Brilliant Ultra Violet™ 737 (BUV737) is
compatible with short-term methanol-based fixation, but should not be
stored in buffers containing methanol for longer than one hour.
There are no specifications
There are no report
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