Invitrogen CD83 Monoclonal Antibody (HB15e), Super Bright™ 702, 100 Tests, eBioscience™
On demandInvitrogen CD83 Monoclonal Antibody (HB15e), Super Bright™ 702, 100 Tests, eBioscience™
The HB15e monoclonal antibody reacts with human CD83, a 45 kDa transmembrane glycoprotein. CD83, a member of the Ig superfamily, is expressed on cultured dendritic cells, interdigitating, follicular, and circulating dendritic cells as well as some proliferating lymphocytes, and human cell lines express this antigen. While the function of CD83 is unclear, it can serve as a useful marker for mature human blood dendritic cells.
Super Bright 702 is a tandem dye that can be excited with the violet
laser line (405 nm) and emits at 702 nm. We recommend using a 710/50
bandpass filter. Please make sure that your instrument is capable of
detecting this fluorochrome.
When
using two or more Super Bright dye-conjugated antibodies in a staining
panel, it is recommended to use Super Bright Complete Staining Buffer to minimize any non-specific polymer interactions.
Please refer to the datasheet for Super Bright Staining Buffer for more
information.
Light
sensitivity: This tandem dye is sensitive to photo-induced oxidation.
Please protect this vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (100
µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse
Solution for up to 3 days in the dark at 4°C with
minimal impact on brightness and FRET efficiency/compensation. Some
generalizations regarding fluorophore performance after fixation can be
made, but clone specific performance should be determined empirically.
There are no specifications
There are no report
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