Invitrogen CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), PE-Cyanine5, 100 µg, eBioscience™

Invitrogen CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), PE-Cyanine5, 100 µg, eBioscience™

The 53-2.1 monoclonal antibody reacts with mouse CD90.2 also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 53-2.1. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Filtration: 0.2 µm post-manufacturing filtered.