Invitrogen CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), PE-eFluor™ 610, 100 µg, eBioscience™
On demandInvitrogen CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), PE-eFluor™ 610, 100 µg, eBioscience™
The 53-2.1 monoclonal antibody reacts with mouse CD90.2 also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 53-2.1. CD90 is involved in regulation of adhesion and signal transduction by T cells.
Light sensitivity: This tandem dye is sensitive photo-induced oxidation.
Please protect this vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (100 µL cell
sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4°C with minimal impact on
brightness and FRET efficiency/compensation. Some generalizations
regarding fluorophore performance after fixation can be made, but clone
specific performance should be determined empirically.
PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits
at 607 nm. We recommend using a 610/20 band pass filter (equivalent to
PE-Texas Red). Please make sure that your instrument is capable of
detecting this fluorochrome.
Light
sensitivity: This tandem dye is sensitive to photo-induced oxidation.
Please protect this vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (100
µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse
Solution for up to 3 days in the dark at 4°C with
minimal impact on brightness and FRET efficiency/compensation. Some
generalizations regarding fluorophore performance after fixation can be
made, but clone specific performance should be determined empirically.
Filtration: 0.2 µm post-manufacturing filtered.
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