Invitrogen CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), Super Bright™ 645, 100 µg, eBioscience™
On demandInvitrogen CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), Super Bright™ 645, 100 µg, eBioscience™
The 53-2.1 monoclonal antibody reacts with mouse CD90.2 also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 53-2.1. CD90 is involved in regulation of adhesion and signal transduction by T cells.
Super Bright 645 is a tandem dye that can be excited with the violet
laser line (405 nm) and emits at 645 nm. We recommend using a 660/20
bandpass filter. Please make sure that your instrument is capable of
detecting this fluorochrome.
When
using two or more Super Bright dye-conjugated antibodies in a staining
panel, it is recommended to use Super Bright Complete Staining Buffer to minimize any non-specific polymer interactions.
Please refer to the datasheet for Super Bright Staining Buffer for more
information.
Light
sensitivity: This tandem dye is sensitive to photo-induced oxidation.
Please protect this vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (100 µL
of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse
Solution for up to 3 days in the dark at 4 degrees C
with minimal impact on brightness and FRET efficiency/compensation.
Some generalizations regarding fluorophore performance after fixation
can be made, but clone specific performance should be determined
empirically.
There are no specifications
There are no report
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