Invitrogen™ CellEvent™ Senescence Green Detection Kit, 25 µL

Invitrogen™ CellEvent™ Senescence Green Detection Kit, 25 µL

The CellEvent Senescence Green Detection Kit consists of a fluorescent probe and optimized buffer that enable the image-based detection of senescent cells. The fluorescein-based probe contains two galactoside moieties, making it a target for β-galactosidase. Activation of β-galactosidase is commonly used as a biomarker for senescent cells. This hydrolase enzyme resides in lysosomes and converts β-galactosides into monosaccharides under acidic pH conditions. The enzymatically cleaved product is retained within the cell due to covalent binding of intracellular proteins and emits a fluorogenic signal that has absorption/emission maxima of 490/514 nm.

Features

• Reliable and quick fluorescent detection of senescent cells
• Greater sensitivity than traditional colorimetric x-gal
•  Fluorescent signal is well retained and detected using standard Alexa Fluor 488/FITC filter sets
• Multiplex-enabled—fluorescent senescence probe can be multiplexed
• For Use With (Equipment): Fluorescence Microscope, High Content Instrument