Invitrogen™ CellLight™ Synaptophysin-RFP, BacMam 2.0

Invitrogen™ CellLight™ Synaptophysin-RFP, BacMam 2.0

CellLight™ Synaptophysin-RFP, BacMam 2.0, provides an easy method for the labeling of synaptophysin with Red Fluorescent Protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning. This ready-to-use construct is transduced/transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to synaptophysin. You can observe synaptophysin-RFP behavior in live cells using a fluorescent imaging system and standard TRITC/RFP filter set. Study synapses in action in live neurons with minimum cellular toxicity or cellular disruption associated with neurotracers. In addition, you can co-transduce/transfect more than one CellLight™ reagent and label live cells with multiple tracking or tracing dyes to image dynamic cellular processes. Cells expressing CellLight™ constructs can also be fixed with formaldehyde for multiplexing with antibodies using immunocytochemical techniques

Features

• For Use With (Equipment): Confocal Microscope, Fluorescence Microscope
• SubCellular Localization: Synaptic Junctions