Invitrogen™ CIAP (Calf Intestinal Alkaline Phosphatase), 1 U/µL

Invitrogen™ CIAP (Calf Intestinal Alkaline Phosphatase), 1 U/µL

Calf Intestinal Alkaline Phosphatase (CIAP) is a phosphomonoesterase that removes 3´ and 5´ phosphates from DNA and RNA

Features

• Concentration: 1 unit/µL
• Applications: Dephosphorylation of 5´-phosphorylated termini of vector DNA to prevent self-ligation (1). Dephosphorylation of 5´ termini of nucleic acids prior to forward reaction with kinase.
• Source: Purified from calf intestinal mucosa.
• Performance and Quality Testing: Endodeoxyribonuclease, 3´ exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency measured in a transformation assay.
• Unit Definition: One unit hydrolyzes 1 µmol of 4-nitrophenyl phosphate in 1 min. at 37°C.
• Unit Reaction Conditions: 1 M diethanolamine buffer, 10 mM 4-nitrophenyl phosphate, 0.25 mM MgCl2 (pH 9.8) in 900 µl for 10 min. at 37°C

Contents

• Calf Intestinal Alkaline Phosphatase (1 unit/µL) is supplied with a vial of 10X dephosphorylation buffer [500 mM Tris-HCl (pH 8.5) 1 mM EDTA]
• vial of dilution buffer [50% glycerol, 25 mM Tris-HCl (pH 7.6)
• 1 mM MgCl2
• 0.1 mM ZnCl2