Invitrogen™ Click-iT™ EdU Proliferation Assay for Microplates

Invitrogen™ Click-iT™ EdU Proliferation Assay for Microplates

The Click-iT EdU Proliferation Assay for Microplates provides a more simplified and robust method for the measurement of mammalian cell proliferation compared to traditional BrdU-based methods. After incorporation of EdU (a modified thymidine analogue) into newly synthesize DNA, horse radish peroxidase (HRP) is covalently attached via the highly specific click reaction. Amplex UltraRed Reagent, an HRP substrate, is then added and the resulting fluorescence signal detected. Because of the highly specific attachment of HRP to EdU, this assay results in highly sensitive and reliable measurement of mammalian cell proliferation in a microplate format

Features

• Improved performance—direct conjugation of HRP to incorporated EdU improves assay sensitivity and accuracy
• Increased reliability—assay format results in low well-to-well variability
• Simplified protocol—results in reduced time to data; easier to follow protocol
• Multiplex enabled—assay can be easily multiplexed with other cell health reagents resulting in more data per sample