Invitrogen™ Click-iT™ Plus Alexa Fluor™ 647 Picolyl Azide Toolkit

Invitrogen™ Click-iT™ Plus Alexa Fluor™ 647 Picolyl Azide Toolkit

Click-iT® Plus Alexa Fluor® picolyl azide toolkits contain everything you need to perform copper-catalyzed click reactions with compounds that are sensitive to copper, while retaining all of the benefits of the standard azide/alkyne click reaction. Picolyl azides react efficiently with chelated copper, so free copper in the click reaction can be minimized, protecting against undesired copper side reactions with proteins (e.g., GFP, RPE), nucleic acids (e.g., RNA, oligos), and even small molecules (e.g., phalloidin).

Features

• Flexible—optimize your own Click–iT® reaction for the detection or labeling of any alkyne-containing biomolecule in vitro or in cells and tissue samples
• Bright and sensitive—superior fluorescent Alexa Flour® dyes combined with the efficiency of picolyl azide
• Convenient—contains all the buffers and reagents needed to create the optimal click reaction

Contents

• Alexa Fluor™ dye-picolyl azide: 1 x 100 μg 
• Click-iT™ reaction buffer: 1 x 4 mL 
• Copper (II) sulfate: 1 x 1.5 mL 
• Copper protectant: 1 x 500 μL 
• Click-iT™ buffer additive