Invitrogen™ CM-H2DCFDA (General Oxidative Stress Indicator)
On demand
Order number :
C6827
Invitrogen™ CM-H2DCFDA (General Oxidative Stress Indicator)
CM-H2DCFDA is a chloromethyl derivative of H2DCFDA, useful as an indicator for reactive oxygen species (ROS) in cells. This indicator exhibits much better retention in live cells than H2DCFDA. CM-H2DCFDA passively diffuses into cells, where its acetate groups are cleaved by intracellular esterases and its thiol-reactive chloromethyl group reacts with intracellular glutathione and other thiols. Subsequent oxidation yields a fluorescent adduct that is trapped inside the cell, thus facilitating long-term studies
Features
- Product is air sensitive and should be stored under dry argon or nitrogen
- Product may be dissolved in DMSO, DMF, or ethanol for use
- Indicator is cell permeant (cell loading protocols are available in the literature)
- Fluorescence can be monitored using a flow cytometer, fluorometer, microplate reader, or fluorescence microscope, using excitation sources and filters appropriate for fluorescein
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