Invitrogen™ Collibri™ Stranded RNA Library Prep Kit for Illumina™ Systems, with Human/Mouse/Rat rRNA Depletion Kit and UD Indexes, 24 Reactions

Invitrogen™ Collibri™ Stranded RNA Library Prep Kit for Illumina™ Systems, with Human/Mouse/Rat rRNA Depletion Kit and UD Indexes, 24 Reactions

The Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina Systems is designed for robust construction of cDNA libraries for strand-specific RNA sequencing on Illumina next-generation sequencing (NGS) systems. The kit brings together the superior features of SuperScript IV Reverse Transcriptase, Dynabeads magnetic particles, and Platinum SuperFi DNA Polymerase, enabling users to obtain high-quality sequencing-ready libraries.
This Collibri Stranded RNA Library Prep Kit is designed for fast and convenient library preparation starting with sample inputs of 100 ng—1 µg of total human, mouse, or rat RNA. Single-tube adapter ligation and reverse transcription reactions, as well as magnetic bead-based rRNA depletion and library purification steps, enable completion of the entire workflow in approximately 6.5 hours. The kit is suitable for various-quality RNA samples, including FFPE samples.

Features

• Short, automation-friendly workflow— total RNA can be converted to sequencing-ready library within 6.5 hours
• Inert dyes in the reagents—see the progress of library generation without any negative effect on quality
• Superior rRNA removal
• Uniform transcript coverage and high transcript detection sensitivity
• High sensitivity of differential gene expression detection
• >98% strand specificity
• Efficient detection of non-coding RNA
• Preservation of 3‘ end sequence information

Applications

• Gene expression studies
• Alternative splicing analysis
• Non-coding RNA detection and discovery
• Identification of alternative polyadenylation sites
• Gene fusion detection
• Detection of transcript isoforms

Contents

• Collibri Library Prep Kit
• Collibri Library Cleanup Kit