Invitrogen Complement C5a Mouse ELISA Kit, 96 Tests

Invitrogen Complement C5a Mouse ELISA Kit, 96 Tests

The Mouse Complement C5a (HC) ELISA quantitates Ms Complement C5a in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Compelemnt C5a.

Principle of the method

The Mouse Complement C5a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Homogenous (no wash): No
• Interassay CV: <12%
• Intraassay CV: <10%
• Shipping condition: Wet or Dry Ice
• Storage: 2-8°C

Contents

• Mouse C5a Antibody Coated wells, 96-well plate, 1 plate 
• Mouse C5a Biotin Conjugate, 2 vials 
• Mouse C5a Standard, recombinant mouse C5a, 2 vials 
• Wash Buffer Concentrate (20X), 25 mL
• Assay Diluent A (contains 0.09% sodium azide), 30 mL 
• Assay Diluent B (5X), 15 mL 
• Streptavidin-HRP (200X), 0.2 mL 
• TMB Substrate, 12 mL 
• Stop Solution, 8 mL 
• Adhesive Plate Covers, 2