Invitrogen Corticosterone Competitive ELISA Kit, 96 Tests

Invitrogen Corticosterone Competitive ELISA Kit, 96 Tests

The Corticosterone ELISA quantitates coritcosterone in serum, plasma, urine, dried fecal extracts or cell culture medium.

Principle of the method

The Corticosterone Competitive ELISA research-use-only kit is designed to quantitatively measure corticosterone present in serum, plasma, urine, extracted dried fecal samples, and tissue culture media samples. This kit measures total corticosterone in serum and plasma and in extracted fecal samples. A corticosterone stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Please choose the standard range that fits your sample concentrations most appropriately. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A corticosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to corticosterone to each well. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound corticosterone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.

Features 

• Homogenous (no wash): No
• Interassay CV: 7.9%
• Intraassay CV: 5.2%
• Shipping condition: Wet or Dry Ice
• Storage: -20°C

Contents

• Pre-coated 96 well plate
• Standard
• Assay Diluent concentrate
• HRP-Conjugated Detection Antibody
• Wash Buffer
• Chromogen
• Stop Solution
• Adhesive Plate Covers