Invitrogen Cortisol Competitive Human ELISA Kit, 96 Tests

Invitrogen Cortisol Competitive Human ELISA Kit, 96 Tests

The Human Cortisol ELISA quantitates Cortisol in human serum, plasma, urine, saliva, dried fecal extracts or or cell culture medium.

Principle of the method

The Cortisol Competitive ELISA research-use-only kit is designed to quantitatively measure cortisol present in dried fecal extracts, saliva, urine, serum, plasma, and tissue culture media samples. Total cortisol is measured in extracted samples and in serum and plasma, and free cortisol in saliva and urine. A cortisol standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A cortisol-peroxidase conjugate is added to the wells. The binding reaction is initiated by the addition of a monoclonal antibody to cortisol. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound cortisol-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm.

Features  

• Homogenous (no wash): No 
• Interassay CV: 8.1%
• Intraassay CV: 8.8%
• Shipping condition: Wet or Dry Ice 
• Storage: -20°C

Contents 

• Cortisol Standard; 32,000 pg/mL cortisol in a special stabilizing solution, 125 μL
• Assay Buffer Concentrate (5X), 28 mL
• Clear 96-well Plate, 96-well strip-well plate; goat anti-mouse IgG coated, 1 plate
• Cortisol Antibody, 3 mL
• Cortisol Conjugate, 3 mL
• Dissociation Reagent, 1 mL
• Wash Buffer Concentrate (20X), 30 mL
• TMB (Tetramethylbenzidine) Substrate, 11 mL
• Stop Solution; contains 1 M HCl, CAUSTIC, 5 mL
• Plate Sealer, 1