Invitrogen CRP Human ELISA Kit, 96 Tests
On demandInvitrogen CRP Human ELISA Kit, 96 Tests
The Human C-Reactive Protein(Hu CRP) ELISA quantitates Hu CRP in human serum and plasma. The assay will exclusively recognize both natural and recombinant Hu CRP.
Principle of the method
The Human CRP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: 9.7%
- Intraassay CV: 7%
- Shipping condition: Wet Ice
- Storage: 2-8°C
Contents
- Hu CRP Standard, lyophilized; contains 0.1% sodium azide. Refer to vial label for quantity and reconstitution volume, 2 vials
- Standard Diluent Buffer; contains 0.1% sodium azide, 2 × 60 mL
- Antibody Coated Plate, 96-well strip-well plate, 1 plate
- Hu CRP Biotin Conjugate; contains 0.1% sodium azide, 11 mL
- Streptavidin-HRP (100X), 0.150 mL
- Streptavidin-HRP Diluent; contains 3.3 mM thymol, 25 mL
- Wash Buffer Concentrate (25X), 100 mL
- Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL
- Stop Solution, 25 mL
- Plate Covers, adhesive strips, 4
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