Invitrogen CSF2 (GM-CSF) Rat ELISA Kit, 5 x 96 Tests
On demandInvitrogen CSF2 (GM-CSF) Rat ELISA Kit, 5 x 96 Tests
The Rat CSF2 (GM-CSF) ELISA quantitates Rt CSF2 in rat serum, plasma, or
cell culture medium. The assay will exclusively recognize both natural
and recombinant Rt CSF2.
Principle of the method
The
Rat CSF2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay)
is designed to measure the amount of the target bound between a matched
antibody pair. A target-specific antibody has been pre-coated in the
wells of the supplied microplate. Samples, standards, or controls are
then added into these wells and bind to the immobilized (capture)
antibody. The sandwich is formed by the addition of the second
(detector) antibody, a substrate solution is added that reacts with the
enzyme-antibody-target complex to produce measurable signal. The
intensity of this signal is directly proportional to the concentration
of target present in the original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: <12%
- Intraassay CV: <10%
- Shipping condition: Wet or Dry Ice
- Storage: 2-8°C
Contents
- Rat CSF2 Antibody Coated wells, 96-well plate,1 plate
- Rat CSF2 Biotin Conjugate, 2 vials
- Rat CSF2 Standard, recombinant Rat CSF2, 2 vials
- Wash Buffer Concentrate (20X), 25 mL
- Assay Diluent D, 30 mL
- Assay Diluent B (5X), 15 mL
- Streptavidin-HRP (400X), 0.2 mL
- TMB Substrate,12 mL
- Stop Solution, 8 mL
- Adhesive Plate Covers, 2