Invitrogen CSF2 (GM-CSF) Swine ELISA Kit, 10 x 96 Tests

Invitrogen CSF2 (GM-CSF) Swine ELISA Kit, 10 x 96 Tests

The Swine CSF2 (GM-CSF) ELISA quantitates Sw CSF2 in swine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Sw CSF2.
Principle of the method
The Swine CSF2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features  

• Homogenous (no wash): No    
• Interassay CV: <12%    
• Intraassay CV: <10%
• Shipping condition: Wet or Dry Ice
• Storage: 2-8°C

Contents    

• Porcin CSF2 Antibody Coated wells, 96-well plate,1 plate
• Porcin CSF2 Biotin Conjugate, 2 vials
• Porcin CSF2 Standard, recombinant Porcin CSF2, 2 vials
• Wash Buffer Concentrate (20X), 25 mL
• Assay Diluent D, 30 mL
• Assay Diluent B (5X), 15 mL
• Streptavidin-HRP (500X), 0.2 mL
• TMB Substrate,12 mL
• Stop Solution, 8 mL
• Adhesive Plate Covers, 2