Invitrogen Cyclic AMP Competitive ELISA Kit, 96 Tests

Invitrogen Cyclic AMP Competitive ELISA Kit, 96 Tests

The cyclic AMP Competitive ELISA quantitates cAMP in cell or tissue lysates. The assay will exclusively recognize both natural and recombinant cyclic AMP.

rinciple of the method

The cyclic AMP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is inversely proportional to the concentration of target present in the original specimen.

Features 

• Homogenous (no wash): No
• Interassay CV: 7.1%
• Intraassay CV: 6.5%
• Storage: -20°C

Contents

• Antibody coated 96-well plate, 1 plate
• cAMP Antibody, 5 mL
• cAMP-AP Conjugate, 5 mL
• 0.1 M HCl, 27 mL
• Neutralizing Reagent, 5 mL
• Triethylamine* ,2 mL
• Acetic Anhydride*, 1 mL
• cAMP Standard (2,000 pmol/mL), 0.5 mL
• 20X Wash Buffer, 27 mL
• pNpp Substrate Solution, 20 mL
• Stop Solution, 5 mL
• Plate Sealer, 1 each