Invitrogen Cytochrome C Human ELISA Kit, 96 Tests, 10 µL

Invitrogen Cytochrome C Human ELISA Kit, 96 Tests, Sample Volume 10 µL

The Human Cytochrome C ELISA quantitates Hu Cytochrome C in human cell and tissue lysates. Cross reactivity has been observed in mouse and rat cells. The assay will exclusively recognize both natural and recombinant Hu Cytochrome C.

Principle of the method

The Human Cytochrome C solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features  

• Homogenous (no wash): No    
• Interassay CV: 6.7%
• Intraassay CV: 4.6%
• Shipping condition: Wet Ice
• Storage: 2-8°C

Contents    

• Cytochrome c Standard; contains 0.1% sodium azide, 2 vials
• Standard Diluent Buffer; contains 0.1% sodium azide, red dye[1], 25 mL
• Antibody Coated Plate; 96-well plate, 1 plate
• Cytochrome c Biotin Conjugate; contains 0.1% sodium azide, blue dye[1], 11 mL
• Streptavidin‑HRP (100X), 0.15 mL
• HRP Diluent; contains 3.3 mM thymol, yellow dye[1], 25 mL
• Wash Buffer Concentrate (25X), 100 mL
• Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL
• Stop Solution, 25 mL
• Adhesive Plate Covers, 3