Invitrogen Cytochrome C Human Matched Antibody Pair, 10 x 96 Tests

Invitrogen Cytochrome C Human Matched Antibody Pair, 10 x 96 Tests

The Human Cytochrome C Matched Antibody Pair Kit comes with pre-matched antibody pairs, standards, assay buffer, lysis buffer and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human Cytochrome C. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately.

Principle of the method

ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features  

• Homogenous (no wash): No    
• Storage: -20°C

Contents    

• Capture (coating) Antibody
• Detection Antibody
• Standard
• SAV-HRP
• Assay Buffer
• Lysis Buffer