Invitrogen™ Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry, SYTOX Green, APC, 50 Assays

Invitrogen™ Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry, SYTOX Green, APC, 50 Assays

The Dead Cell Apoptosis Kit with Annexin V APC and SYTOX Green detects PS externalization in apoptotic cells during flow cytometry using recombinant annexin V conjugated to red laser-excited allophycocyanin, and dead cells using SYTOX Green nucleic acid stain. Apoptotic cells are detected by annexin V binding to externalized PS. Live cells show little or no fluorescence, apoptotic cells show red fluorescence and very little green fluorescence, and late apoptotic cells show a higher level of red and orange fluorescence. These populations can easily be distinguished using a flow cytometer that has both 488 nm and 633 nm excitation sources (an argon-ion laser and a HeNe laser).

Features

• Superior brightness.
• High binding efficiency, Annexin V conjugates are made from a highly purified cys-annexin, resulting in higher binding efficiency and extremely accurate characterization of the apoptotic process.
• Multi-parametric, Many publications require a minimum of two different ways to identify apoptotic cells. This multi-parametric kit detects phosphatidyl serine (PS) on the cytoplasmic surface of the cell membrane, while membrane integrity is determined via propidium iodide.

Contents

• 1 vial of annexin V, APC conjugate (250 µL).
• 1 vial of SYTOX green stain (100 µL).
• 1 bottle of annexin binding buffer (5X solution, 15 mL).