Invitrogen Decorin (DCN) Mouse ELISA Kit, 5 x 96 Tests

Invitrogen Decorin (DCN) Mouse ELISA Kit, 5 x 96 Tests

The Mouse Decorin (DCN) ELISA quantitates Ms Decorin in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Decorin.

Principle of the method

The Mouse Decorin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features  

• Homogenous (no wash): No    
• Interassay CV: <12%    
• Intraassay CV: <10% 
• Shipping condition: Wet or Dry Ice
• Storage: 2-8°C

Contents    

• Mouse Decorin Antibody Coated wells, 96-well plate, 1 plate 
• Mouse Decorin Biotin Conjugate, 2 vials 
• Mouse Decorin Standard, recombinant mouse decorin, 2 vials 
• Wash Buffer Concentrate (20X), 25 mL 
• Assay Diluent (5X), 15 mL 
• Streptavidin-HRP (300X), 0.2 mL
• TMB Substrate, 12 mL 
• Stop Solution, 8 mL
• Adhesive Plate Covers, 2