Invitrogen™ Dynabeads™ Mouse CD4

Invitrogen™ Dynabeads™ Mouse CD4

Dynabeads™ Mouse CD4 are magnetic beads that provide a robust and versatile tool to isolate or deplete mouse CD4+ T-cells directly from any sample, including tissue digests such as spleen or lymph nodes, whole blood, bone marrow, and mononuclear cells (MNC).

Excellent Yield, Purity, and Viability of Recovered Cells, Dynabeads™ Mouse CD4 are uniform, superparamagnetic beads (4.5 µm diameter) that enable easy isolation of mouse CD4⁺ cells from spleen or lymph nodes, although any single-cell suspension may be used. Dynabeads™ Mouse CD4 beads are coated with a primary monoclonal antibody specific for the CD4 membrane antigen mainly expressed on mouse T helper cells. Dynabeads™ Mouse CD4 binds to CD4⁺ cells during a short incubation period in a tube, after which the bead-bound cells are separated from unbound cells using a magnet. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the isolated CD4⁺ cells.

Ideal for Both Depletion and Positive Isolation for Downstream Molecular Assays, Because of the powerful magnets used for separation, Dynabeads™ Mouse CD4 can be used in viscous samples such as single cell suspensions from tissue, whole blood and bone marrow, and enable efficient depletion of mouse CD4+ cells in about 30 minutes. Dynabeads™ Mouse CD4 will also afford excellent recovery of high-purity, viable cells when used for positive isolation for downstream studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified.

Features

• Rapid isolation of mouse CD4⁺ T-cells from any sample – no columns required.
• Positive isolation for molecular downstream assays or efficient depletion of mouse CD4⁺ T cells.

Contents

• 5 ml Dynabeads™Mouse CD4 (L3T4) coated with anti-L3T4 monoclonal antibody. The L3T4 antigen is expressed on mouse thymocytes and the T helper subsets of mature T cells of all common mouse strains.