Invitrogen™ eBioscience™ Calcium Sensor Dye eFluor™ 514, 1 mg
On demand
Order number :
65-0859-39
Invitrogen™ eBioscience™ Calcium Sensor Dye eFluor™ 514, 1 mg
eFluor™ 514 Calcium Sensor Dye is a membrane-permeable dye used for monitoring changes in intracellular free calcium concentrations in the cell using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Once eFluor™ 514 Calcium Sensor Dye enters the cell, cellular esterases cleave the AM group yielding a membrane-impermeable dye fluorescing at ∼520 nm . eFluor™ 514 Calcium Sensor Dye, like Fluo-3 and Fluo-4, is a commonly used dye among the visible light-excitable calcium indicators but with increased cellular uptake (even at room temperature) and brightness.
- calcium binding affinity: Kd = 232 nM
- eFluor™ 514 Calcium Sensor Dye should be reconstituted in high-quality, freshly opened DMSO.
- Recommended concentration: 2-5 mM.
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