Invitrogen™ eBioscience™ DRAQ5™, 50 µL

Invitrogen™ eBioscience™ DRAQ5™, 50 µL

DRAQ5™ is an anthraquinone dye with high affinity for double-stranded DNA. It is a membrane-permeable dye that can label live or fixed/dead cells. In flow cytometry, this dye can be used to distinguish nucleated and non-nucleated cells. DRAQ5™ can also be used to report nuclear DNA content for ploidy and cell cycle analysis because it binds DNA stoichiometrically. In fluorescent microscopy, it can be used as a nuclear counterstain. DRAQ5™ can be excited by wavelengths from 488 to 647 nm. For microscopy/imaging, 633 or 647 nm sources for excitation are recommended. For flow cytometry, with excitation at 488 nm, this dye can be detected with filter sets designed for PerCP-eFluor™ 710 or PerCP-Cy5.5 (685LP dichroic mirror and 710/50 band pass); with excitation at 633 nm, it can be detected with filter sets designed for APC (660/20 band pass). For cell cycle/DNA analysis applications, a higher wavelength filter is recommended, such as a 710LP or a filter set designed for PE-Cy7 (735LP dichroic mirror and 780/60 band pass), to optimize the CV for the G1 and G2/M peaks. Please make sure that your instrument is capable of detecting this dye.

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