Invitrogen™ eBioscience™ Mouse Regulatory T Cell Staining Kit #1

Invitrogen™ eBioscience™ Mouse Regulatory T Cell Staining Kit #1

The FJK-16s antibody reacts with mouse/rat Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3 a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

FJK-16s cross-reacts with rat Foxp3 This has been demonstrated by intracellular staining of Foxp3 and flow cytometry of rat splenocytes using the same method and reagents as used for mouse tissue. Please note that the CD4 and CD25 antibodies included in this kit only recognize the mouse antigens.

Host: Rat

Isotype: IgG2a

Reactivity/Species: Mouse

Content

• Anti-Mouse CD4 FITC (RM4-5), Store at 4°C in the dark.
• Anti-Mouse CD25 APC (PC61.5), Store at 4°C in the dark.
• Anti-Mouse/Rat Foxp3 PE (FJK-16s), Store at 4°C in the dark.
• Rat IgG2a Isotype Control PE, Store at 4°C in the dark.
• Anti-Mouse CD16/32 (Fc Block) Purified, Store at 4°C in the dark.
• Flow Cytometry Staining Buffer.
• Fixation/Permeabilization Concentrate
• Fixation/Permeabilization Diluent, Store at 4°C. 
• Permeabilization Buffer (10X), Store at 4°C.