Invitrogen EGF Rat ELISA Kit, 5 x 96 Tests
On demandInvitrogen EGF Rat ELISA Kit, 5 x 96 Tests
The Rat Epidermal Growth Factor (Rt EGF) ELISA quantitates Rt EGF in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt EGF.
Principle of the method
The Rat EGF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: <12%
- Intraassay CV: <10%
- Shipping condition: Wet or Dry Ice
- Storage: 2-8°C
Contents
- Rat EGF Antibody Coated wells, 96-well plate, 1 plate
- Rat EGF Biotin Conjugate, 2 vials
- Rat EGF Standard, recombinant rat EGF, 2 vials
- Wash Buffer Concentrate (20X), 25 mL
- Assay Diluent A (contains 0.09% sodium azide), 30 mL
- Assay Diluent B (5X), 15 mL
- Streptavidin-HRP (200X), 0.2 mL
- TMB Substrate, 12 mL
- Stop Solution, 8 mL
- Adhesive Plate Covers, 2
There are no specifications
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