Invitrogen Eotaxin Human ELISA Kit, 96 Tests
On demandInvitrogen Eotaxin Human ELISA Kit, 96 Tests
The Human Eotaxin ELISA quantitates Hu Eotaxin in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Eotaxin.
Principle of the method
The Human Eotaxin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: 4.9%
- Intraassay CV: 4.2%
- Shipping condition: Wet Ice
- Storage: 2-8°C
Contents
- Hu Eotaxin Standard; lyophilized. Refer to vial label for quantity and reconstitution volume, 2 vials
- Standard Diluent Buffer; contains 7.7 mM sodium azide, 25 mL
- Hu Eotaxin Antibody‑Coated Wells, 96-well strip-well plate, 1 plate
- Hu Eotaxin Biotin Conjugate; contains 7.7 mM sodium azide, 11 mL
- Streptavidin‑Peroxidase (HRP) (100X); contains 0.04% Proclin™ 300, 3.3 mM thymol, 0.125 mL
- Incubation Buffer; contains 7.7 mM sodium azide, 11 mL
- Streptavidin‑Peroxidase (HRP) Diluent; contains 0.04% Proclin™ 300 and 3.3 mM thymol, 25 mL
- Wash Buffer Concentrate (25X), 100 mL
- Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL
- Stop Solution, 25 mL
- Plate Covers, adhesive strips, 3
There are no specifications
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