Invitrogen Epiandrosterone ELISA Kit, 96 Tests
On demandInvitrogen Epiandrosterone ELISA Kit, 96 Tests
The Epiandrosterone Competitive ELISA quantitates epiandrosterone in serum, plasma, urine, saliva, dried fecal extracts or cell culture medium.|Principle of the method: The Epiandrosterone Competitive ELISA research-use-only kit is designed to quantitatively measure epiandrosterone independent of species. An epiandrosterone standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with anti-rabbit IgG antibodies. Anti-rabbit epiandrosterone antibodies and epiandrosterone-peroxidase conjugate is added to the wells. The epiandrosterone-peroxidase conjugate and any epiandrosterone in the sample will compete to bind to the anti-rabbit epiandrosterone antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound epiandrosterone-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of epiandrosterone present in the sample.|Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Features
- Homogenous (no wash): No
- Interassay CV: 16%%
- Intraassay CV: 8%
- Shipping condition: Wet or Dry Ice
- Storage: -20°C
Contents
- Coated Clear 96-Well Plate, clear plastic microtiter plate coated with goat anti-rabbit IgG, 1 plate
- Epiandrosterone Standard; 1,000 ng/mL, 125 μL
- Epiandrosterone Antibody, 3 mL
- Epiandrosterone Conjugate, 3 mL
- Assay Buffer Concentrate (5X), 28 mL
- Wash Buffer Concentrate (20X), 30 mL
- TMB (Tetramethylbenzidine) Substrate, 11 mL
- Stop Solution; contains 1 M HCl, CAUSTIC, 5 mL
- Plate Sealer, 1
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