Invitrogen™ Epidermal Growth Factor, Biotinylated, complexed to Alexa Fluor™ 647 Streptavidin (Alexa Fluor™ 647 EGF complex)

Invitrogen™ Epidermal Growth Factor, Biotinylated, complexed to Alexa Fluor™ 647 Streptavidin (Alexa Fluor™ 647 EGF complex)

We offer several conjugates of epidermal growth factor that are useful for the detection of EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. The conjugates incorporating fluorescein (FITC), Oregon Green® 514, and tetramethylrhodamine were constructed with the dye directly attached to EGF. The conjugates of EGF with Alexa Fluor® dyes and Texas Red™ dye are complexes of the dye–streptavidin molecule and biotinylated EGF. The biotinylated conjugates utilize biotin-XX, which contains a long spacer arm designed to enhance the probe’s affinity for the EGF receptor

Features

• EGF molecule: 53 amino acids, MW=6,045 Da
• Label (Ex/Em): Biotin-XX complexed with Alexa Fluor® 647 streptavidin (∼650/665 nm)
• Number of fluorophore molecules on each EGF molecule: ∼2–3
• Fluorescence is typically monitored using a flow cytometer, fluorescence microscope, or fluorimeter