Invitrogen™ Epidermal Growth Factor, Biotinylated, complexed to Texas Red™ Streptavidin (Texas Red™ EGF complex)

Invitrogen™ Epidermal Growth Factor, Biotinylated, complexed to Texas Red™ Streptavidin (Texas Red™ EGF complex)

We offer several conjugates of epidermal growth factor that are useful for the detection of EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. The conjugates incorporating fluorescein (FITC), Oregon Green® 514, and tetramethylrhodamine were constructed with the dye directly attached to EGF. The conjugates of EGF with Alexa Fluor® dyes and Texas Red™ dye are complexes of the dye–streptavidin molecule and biotinylated EGF. The biotinylated conjugates utilize biotin-XX, which contains a long spacer arm designed to enhance the probe’s affinity for the EGF receptor

Features

• EGF molecule: 53 amino acids, MW=6,045 Da
• Label (Ex/Em): Biotin-XX complexed with Texas Red™ streptavidin (∼595/615 nm)
• Number of fluorophore molecules on each EGF molecule: ∼2–3
• Fluorescence is typically monitored using a flow cytometer, fluorescence microscope, or fluorimeter