Invitrogen FGF1 (FGF alpha) Human ELISA Kit, 10 x 96 Tests
On demandInvitrogen FGF1 (FGF alpha) Human ELISA Kit, 10 x 96 Tests
The Human Fibroblast Growth Factor 1 (Hu FGF1/FGFα) ELISA quantitates Hu FGF1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu FGF1.
Principle of the method
The Human FGF1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: <12%
- Intraassay CV: <10%
- Shipping condition: Wet or Dry Ice
- Storage: 2-8°C
Contents
- Human FGF1 Antibody Coated wells, 96-well plate, 10 plate
- Human FGF1 Biotin Conjugate, 20 vials
- Human FGF1 Standard, recombinant human FGF1, 20 vials
- Wash Buffer Concentrate (20X), 12 x 25 mL
- Assay Diluent (5X), 10 x 15 mL
- Streptavidin-HRP (500X), 10 x 0.2 mL
- TMB Substrate, 10 x 12 mL
- Stop Solution, 10 x 8 mL
- Adhesive Plate Covers, 20
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